Efektivitas Pasta Gigi dengan Kandungan Ekstrak Biji Kopi Robusta (Coffea canephora) dalam Memodulasi Adhesi Streptococcus mutans Terhadap Monosit
(Effectiveness of Toothpaste with Robusta Coffee Bean Extract (Coffea canephora) in Modulating Streptococcus mutans Adhesion to Monocytes)
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Anindya Farida Ayu Afiffah
Fakultas Kedokteran Gigi, Universitas Jember, Indonesia
Peni Pujiastuti
Bagian Periodonsia, Fakultas Kedokteran Gigi, Universitas Jember, Indonesia
Depi Praharani
Bagian Periodonsia, Fakultas Kedokteran Gigi, Universitas Jember, Indonesia
DOI: https://doi.org/10.19184/stoma.v22i1.53697
ABSTRACT
S. mutans can invade blood vessels which will trigger a monocyte response with adhesion. Excessive adhesion of S. mutans to monocytes can cause prolonged inflammation and tissue damage so adhesion must be modulated. - The purpose of this study was to examine the ability of toothpaste with robusta coffee bean extract concentrations of 0.78%; 1.56%; 3.12%; and 6.25% in modulating - the adhesion of S. mutans to monocytes. This - is an in vitro laboratory experiment using the post-test only control group design. The sample was divided into 2 groups: Control group (placebo paste) and the treated group (toothpaste containing 0.78%; 1.56%; 3.12%; and 6.25% of robusta coffee bean extract). Placebo paste and toothpaste with robusta coffee bean extract were diluted using RPMI (1:10) before being applied to each sample. Monocyte isolate was incubated with placebo paste and toothpaste with robusta coffee bean extract for 2.5 hours, then - exposured to S. mutans for 4 hours. The adhesion index -was determined by calculating the average number of S. mutans attached to 100 monocytes. The results P<0,05 showed that there were significant differences between the treatment groups. The highest adhesion index was found at a concentration of 0.78% with 5,55% and the lowest at a concentration of 6.25% showed 1,37%. The conclusion of this study based on is that toothpaste with robusta coffee bean extract concentrations of 0.78%; 1.56%; 3.12%; and 6.25% were able to modulate by inhibiting the adhesion of S. mutans to monocytes with the most effective concentration is 6.25%.
Published
27-3-2025
Issue
Vol. 22 No. 1 2025: Jurnal Stomatognatic
Pages
54-58
License
Copyright (c) 2025 Jurnal Stomatognatic
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